Cultivation of Human Induced Pluripotent Stem Cells with Controlled Aggregate Size and Geometrical Arrangement by Inverting Microwell Array Chip
نویسندگان
چکیده
This paper reports a novel cell culture chip, namely “inverting microwell array chip”, for cultivation of human induced pluripotent stem cells with controlled microenvironment. Our “inverting microwell array chip” comprises a lower hydrogel microwell array and an upper polystyrene (PS) culture surface. We demonstrate the formation of uniform human induced pluripotent stem cell aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the PS surface.
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